Dig-high prime dna labeling and detection starter kit ii manual

 · The segment was tag labeled with DIG-high prime (Roche, USA) as probe. The prehybridization, hybridization, washing, detection and exposure were operated according to the manual of DIG High Prime DNA Labeling and Detection Starter Kit II (Roche, Indianapolis, IN, USA). Virus inoculation and resistance evaluation. DIG High Prime DNA Labeling and Detection Starter Kit II y Version 14 Content version: August Random primed DNA labeling with digoxigenin-dUTP, alkali-labile, and chemiluminescent detection with CSPD, ready-to-use Cat. No. 11 Kit for 12 labeling reactions of 10 ng to 3 g DNA and detection of 24 blots of cm2.  · The candidate disruptants ∆Mowhi2 were verified with PCR assay and further Southern blot assay with a DIG-High Prime DNA Labeling and Detection Starter Kit (Roche). To generate the complemented strains Mowhi2 c, a genomic gene sequence driven by the MoWHI2 native promoter was amplified and ligated into Xma I/ Hin dIII-digested pFGL

Firstly, the prepared labeled probe was introduced the EcoR I stick in the end of probe for 3'-end labeling, and then was performed the probe labeling and detecting the signals of EMSA with the relatively cheap DIG High Prime DNA Labeling and Detection Starter Kit II Rohe. By adjusting the experiment parameters, the successful result was obtained. Procedures for Labeling DNA, RNA, and Oligonucleotides with DIG, Biotin, or Fluorochromes I. Random primed labeling of ds DNA with DIG-, Biotin- or Fluorescein-High Prime reaction mix II. PCR labeling of ds DNA with the PCR DIG Probe Synthesis Kit or. DIG-High Prime has enzyme and nucleotide mixture for rapid random-primed labeling of DNA with DigoxigenindUTP. In this method, the complementary DNA strand of denatured DNA is synthesized by Klenow polymerase using the 3′-OH termini of the random oligonucleotides as primers.

DIG High Prime DNA Labeling and Detection Starter Kit II, and DIG cDNA Synthesis kit according to the manufacturer's instructions, and a DNA probe. labeled with DIG following the instructions in the DIG. High Prime DNA labeling and Detection Starter Kit I. (Roche Molecular Biochemicals, Indianapolis. Read independent reviews on DIG-High Prime DNA Labeling and Detection Starter Kit I from Roche Applied Science - a member of the Roche Group on.